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the clean air conditioning system of the clean room gmp program ensures the required cleanliness by filtering the air. the biological clean room needs to simultaneously control the number of dust particles in the room and the floating bacteria and sedimentation bacteria. if the necessary disinfection measures are not taken, it is difficult to control the number of microorganisms in the room for a long time only by purifying the three-stage filtration process of the air conditioning system. the secondary microbial contamination problem of purifying air conditioning systems has received more and more attention. the air conditioning treatment equipment will inevitably accumulate bacteria and dust during operation. once the system stops running, the high temperature and high humidity environment will provide favorable conditions for microbial reproduction. a large part of the mass-produced microorganisms and their released harmful substances, harmful metabolites, corpses and debris are unstoppable by the filter, which will cause secondary pollution of microorganisms. also in the clean room, due to the activities of personnel and the entry and exit of articles, it is impossible to purify the air conditioning system to completely bring all the dust and microorganisms out of the clean room, and some of the microorganisms will be in the clean room.
continue to breed and cause pollution.
for the biosesafety clean room, due to the high risk of the research object, it is the focus of all work to prevent the pollution of the workers in the laboratory and the environment outside the laboratory. the purification air conditioning system must have corresponding disinfection measures to ensure disinfection of operation and maintenance personnel biological clean room and its purification air conditioning system
proper disinfection and sterilization measures and methods for air-conditioning purification systems are the key to ensuring that biosesafety of clean systems meets the requirements for use. combined with the characteristics of ozone disinfection and sterilization, this paper focuses on the application of ozone (o) in air conditioning purification systems.
3 clean air conditioning ozone disinfection sterilization (o) 3 safety. therefore, the purification of the air conditioning system and the clean room must be taken properly
disinfection measures and methods are required to ensure that the biological clean room and the biosesafety clean room meet the requirements for use. traditional biological clean rooms are usually sterilized with chemical reagents. this method has a large medicinal taste and is difficult to discharge naturally. it requires an air conditioner to replace the fresh air for a long time. at the same time, there is also the problem of secondary pollution. the remaining drugs are directly discharged into the atmosphere, causing pollution to the surrounding environment. such as formaldehyde fumigation, troublesome operation, long fumigation time, secondary pollutants, have certain harm to the human body. it takes an hour to make a fumigation, and the residue adheres to the clean wall and the surface of the device and needs to be erased. within a few days after disinfection, the number of suspended particles will increase, which will cause some damage to the high-efficiency filter. more importantly, such disinfection and sterilization methods are difficult to disinfect the air treatment unit, the exhaust high-efficiency filter, the high-grade biosesafety clean room requirements, and the air duct. in recent years, more and more ozone disinfection methods have been applied. the dust-free workshop gmp plan scientific research shows that ozone has a strong bactericidal effect. ozone has unstable molecular structure at normal temperature and pressure, and quickly decomposes itself into oxygen and a single oxygen atom; the latter has strong oxidative properties against bacteria. oxygen oxidation decomposes the enzymes necessary to oxidize glucose inside the bacteria, destroying its cell membrane and killing it. the excess oxygen atoms recombine themselves into ordinary oxygen atoms without any toxic residues, so no pollution